Proces umjetne sintetizacije artemisinina nije samo kompliciran, već također ima nizak prinos i visoku toksičnost. Stoga se trenutna ponuda artemisinina na tržištu uglavnom dobiva iz biljke Artemisia annua. Glavne metode ekstrakcije aktivnih sastojaka Artemisia annua uključuju dekokciju, ekstrakciju refluksom, ekstrakciju organskim otapalom, ultrazvučnu ekstrakciju, mikrovalnu ekstrakciju i CO2 superkritičnu ekstrakciju.
The reflux extraction method is one of the common methods for extracting plant active ingredients. Using ethanol as the organic solvent for reflux extraction is easy to operate, has high extraction rate, and does not pollute the environment. When using reflux extraction to extract active ingredients from plants, solvent concentration, solid-liquid ratio, extraction temperature, extraction time, etc. are important factors that affect extraction efficiency. Wang Lifang and other scholars have shown that the optimal extraction process conditions for the active ingredients of Artemisia annua are 55% ethanol concentration, 95°C extraction temperature, 2 h extraction time, and a solid-liquid ratio of 1:8, with ethanol concentration and extraction temperature being the main influencing factors. , extraction time and solid-liquid ratio are secondary influencing factors. In this experiment, ethanol was selected as the extraction solvent, and the ethanol concentration, solid-liquid ratio, extraction temperature, and extraction time for extracting the active ingredients of Artemisia annua were optimized. The results show that the order of factors in extracting active ingredients of Artemisia annua by reflux extraction is ethanol concentration>extraction temperature>solid-liquid ratio>vrijeme ekstrakcije, a optimalni parametri proizvodnog procesa su koncentracija od 65%, omjer kruto-tekuće 1:8, temperatura ekstrakcije bila je 80 stupnjeva, a vrijeme ekstrakcije 4 sata, što je bilo slično rezultatima prethodnih istraživanja.